Smart proteolysis samplers for pre‐lab bottom‐up protein analysis – Performance of on‐paper digestion compared to conventional digestion

Here the relation between digestion of proteins by trypsin covalently bound to paper and in-solution is investigated. The acting on bound. A concentration 0.5% (w/v) results in highest activity all concentrations tested. Additionally, it can be seen that on-paper has retained approx. 50% its activity. Unlike in-solution, stability smart proteolysis samplers was regarded stable for at least four months when kept refrigerated. Autolysis very small trypsin: less than 2% compared trypsin. Proteomic analysis diluted human serum showed more protein identifications (214) digestions (76). Also, higher coverage obtained. Those identified after with no or few disulfide bonds seem have similar sequence coverages those digestion. Smart allow determination 70–75 without performing overnight All all, shows retain high proteolytic a alternative conventional digestions. In this way, show their feasibility pre-lab sample preparation.